anti-Pig (Porcine) TCF4 antibody for Western Blotting

Recommended TCF4 Antibody (supplied by: Log in to see )

Antigen
Transcription Factor 4 (TCF4) Antibodies
  • TCF7L2
  • TCF4
  • XE2
  • Xtcf-4
  • Xtcf4
  • e2-A
  • tcf-4
  • E2-2
  • ITF-2
  • ITF2
  • PTHS
  • SEF-2
  • SEF2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • TCF-4
  • bHLHb19
  • 5730422P05Rik
  • ASP-I2
  • E2.2
  • ITF-2b
  • ME2
  • MITF-2A
  • MITF-2B
  • TFE
  • Tcf-4
  • TCF4B
  • TCF4E
  • Tcf4
  • mTcf-4B
  • mTcf-4E
  • GE2
  • transcription factor 7-like 2 (T-cell specific, HMG-box)
  • transcription factor 4
  • transcription factor 7 like 2, T cell specific, HMG box
  • TCF7L2
  • tcf4
  • TCF4
  • Tcf4
  • Tcf7l2
  • LOC396347
Epitope
N-Term
16
12
7
6
4
4
3
3
3
2
1
1
1
1
Reactivity
Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
85
24
13
5
4
4
3
2
2
1
1
1
Host
Rabbit
55
19
11
Clonality
Polyclonal
Conjugate
This TCF4 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
79
42
22
20
14
12
9
4
2
2
1
1
1
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Catalog No. ABIN2792580
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1494933 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN214151 IHC (p) WB Rabbit IgG AA 73-122 Log in to see Polyclonal
1 ABIN1585796 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

General

Antigen Transcription Factor 4 (TCF4) Antibodies
Epitope N-Term
(16), (12), (7), (6), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(85), (24), (13), (5), (4), (4), (3), (2), (2), (1), (1), (1)
Host Rabbit
(55), (19), (11)
Clonality
Polyclonal
Conjugate This TCF4 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(79), (42), (22), (20), (14), (12), (9), (4), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-TCF4 Antibody

Target Details TCF4 Application Details Handling References for anti-TCF4 antibody (ABIN2792580) Images
Sequence DGTPYDHMTS RDLGSHDNLS PPFVNSRIQS KTERGSYSSY GRESNLQGCH
Cross-Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against TCF4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TCF4

Target Details TCF4

Product Details anti-TCF4 Antibody Application Details Handling References for anti-TCF4 antibody (ABIN2792580) Images back to top
Antigen
Alternative Name TCF4 (TCF4 Antibody Abstract)
Background TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts, however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.
Alias Symbols: E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, PTHS, ITF-2, SEF-2, TCF-4, bHLHb19
Protein Interaction Partner: GOLGA8F, REXO1L6P, FAM74A4, GOLGA8EP, C9orf171, RAB41, SEC14L4, NEK8, ZDHHC24, MSRB3, FERD3L, PPP1R18, NUDT10, PATE1, TMEM213, KLC3, ZNF417, NEU4, NR2C2AP, POLR1A, SZT2, EPB41L3, MAPKBP1, SLC4A1AP, FRS3, NEK6, GLRX3, MAD2L2, BCAS2, TSSC4, CHAF1A, POLR1C,
Protein Size: 667
Molecular Weight 71 kDa
Gene ID 6925
NCBI Accession NM_003199, NP_003190
UniProt P15884
Research Area Stem Cells, Wnt, Transcription Factors
Pathways WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus

Application Details

Product Details anti-TCF4 Antibody Target Details TCF4 Handling References for anti-TCF4 antibody (ABIN2792580) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 667 AA

Restrictions For Research Use only

Handling

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details References for anti-TCF4 antibody (ABIN2792580) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TCF4 antibody (ABIN2792580)

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling Images back to top
Background publications

Raurell, Castaño, Francí, García de Herreros, Duñach: "Presenilin-1 interacts with plakoglobin and enhances plakoglobin-Tcf-4 association. Implications for the regulation of beta-catenin/Tcf-4-dependent transcription." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1401-11, 2006

Images

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling References for anti-TCF4 antibody (ABIN2792580) back to top
Supplier Images
Western Blotting (WB) image for anti-Transcription Factor 4 (TCF4) (N-Term) antibody (ABIN2792580) anti-Transcription Factor 4 (TCF4) (N-Term) antibody