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anti-Dog (Canine) WNT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT2 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family Member 2 (WNT2) Antibodies
  • 2610510E18Rik
  • CG1916
  • D-wnt-2
  • Dm-2
  • Dm DWnt2
  • Dmel\\CG1916
  • Dwnt-2
  • DWnt-2
  • dWnt2
  • DWnt2
  • INT1L1
  • Int1l1
  • int1l1
  • Irp
  • IRP
  • irp
  • Mirp
  • Wnt
  • Wnt-2
  • wnt-2
  • WNT2
  • wnt2
  • Wnt2a
  • Xwnt-2
  • Xwnt2
  • ZfWnt2
Epitope
AA 240-290
12
8
4
3
2
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
55
30
22
12
Host
Rabbit
58
Clonality
Polyclonal
Conjugate
This WNT2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
34
10
10
9
5
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN762896
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985059 ABIN762898 IHC (p) WB Biotin Rabbit IgG AA 240-290 Log in to see Polyclonal
0.0008985059 ABIN762905 IHC (p) WB HRP Rabbit IgG AA 240-290 Log in to see Polyclonal

Top referenced anti-WNT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-WNT2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 32 Antibodies 20 Antibodies 12 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 10 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 5 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family Member 2 (WNT2) Antibodies
Epitope AA 240-290
(12), (8), (4), (3), (2), (2), (2), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(55), (30), (22), (12)
Host Rabbit
(58)
Clonality
Polyclonal
Conjugate This WNT2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(34), (10), (10), (9), (5), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT2 Antibody

Target Details WNT2 Application Details Handling References for anti-WNT2 Antibody (ABIN762896) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human WNT2
Isotype IgG

Target Details WNT2

Product Details anti-WNT2 Antibody Application Details Handling References for anti-WNT2 Antibody (ABIN762896) Images back to top
Antigen
Alternative Name WNT2 (WNT2 Antibody Abstract)
Background

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Subcellular location: Secreted


Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2
Gene ID 7472
UniProt P09544
Research Area Cancer, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-WNT2 Antibody Target Details WNT2 Handling References for anti-WNT2 Antibody (ABIN762896) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-WNT2 Antibody Target Details WNT2 Application Details References for anti-WNT2 Antibody (ABIN762896) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-WNT2 Antibody (ABIN762896)

Product Details anti-WNT2 Antibody Target Details WNT2 Application Details Handling Images back to top
Product cited in:

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, 2014

Images

Product Details anti-WNT2 Antibody Target Details WNT2 Application Details Handling References for anti-WNT2 Antibody (ABIN762896) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Formalin-fixed and paraffin embedded human placenta labeled with Anti-WNT2 Polyclonal...
Immunohistochemistry (IHC) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Immunohistochemistry (IHC) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Western Blotting (WB) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown i...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded ca...
Western Blotting (WB) image for anti-WNT2 antibody (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Mouse brain lysates probed with Anti-WNT2 Polyclonal Antibody, Unconjugated at 1:500...
'Independent Validation' Badge
Lot Number 131105
Method validated Immunohistochemistry
Positive Control Human glioma tissue
Negative Control Human brain
Notes Signal was detected in positive control tissue and not in negative control tissue.
Primary Antibody
  • Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
  • Catalog number: ABIN762896
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131105
Isotype Control Antibody
Secondary Antibody
  • Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Batch number: 10082183
Controls
  • Positive control: Human Glioma (specimen known to contain the target protein) from HTSR’s Human Tissue Bank.
  • Negative Control: Human Normal Brain (specimen known to not contain the target protein) from HTSR’s Human Tissue Bank.
  • Primary antibody isotype control: Human Glioma treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Human Glioma treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed by hand.
  • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
  • Sections were rinsed in de-ionized water for 5 min at RT.
  • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
  • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
  • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
  • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
  • Sections were washed x 1 in Distilled Water.
  • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were blued in Ammonium Hydroxide for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
Experimental Notes
  • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
  • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
Validation Images
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 1: Human brain glioma tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 2: Human normal brain tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 3: Human brain glioma tissue stained with isotype control antibody (brown) and...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 4: Human brain glioma tissue stained with secondary only (brown) and counterst...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 5: Human brain tissue with Parkinson's morphology stained with anti-Wnt2 (brow...