anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) Antibodies

CTCF is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains.

list all antibodies Gene Name GeneID UniProt
Anti-Mouse CTCF CTCF 13018 Q61164
Anti-Human CTCF CTCF 10664 P49711
Anti-Rat CTCF CTCF 83726 Q9R1D1
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Top anti-CTCF Antibodies at antibodies-online.com

Showing 10 out of 99 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated ChIP, WB Chromatin Immunoprecipitation (ChIP) Using CTCF antibody - N-terminal region  and HCT116 Cells WB Suggested Anti-CTCF Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:312500  Positive Control:  Transfected 293T 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB Host: Rabbit Target Name: CTCF Sample Tissue: Mouse Spleen Antibody Dilution: 1ug/ml Host: Rabbit Target Name: CTCF Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated ChIP, WB WB Suggested Anti-CTCF Antibody Titration:  0.2-1 ug/ml  Positive Control:  Transfected 293T Chromatin Immunoprecipitation (ChIP) Using CTCF antibody - N-terminal region  and HCT116 Cells 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ChIPSeq, ChIP, ICC, IF, WB CTCF antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 8 µl of CTCF antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the SCN4A gene (site A or site B). Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG (arbitrarily assigned a value of 1) using the ddCT method. CTCF antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human B cell lymphoma cell line (4.5 million cells) and 5 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows CTCF binding across a 1.2 million bp region on chromosome 7. 100 μL Log in to see 1 to 2 Days
$507.50
Details
Human Rabbit Un-conjugated IHC (p), WB Anti-CTCF Picoband antibody, IHC(P) IHC(P): Human Lung Cancer Tissue Anti-CTCF Picoband antibody, IHC(P) IHC(P): Mouse Intestine Tissue 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated ELISA, WB Anti-CTCF in Western Blot using Immunochemical affinity purified Anti-CTCF antibody shows detection of CTCF in H929 nuclear cell lysates. Primary antibody was diluted to 1.2 µg/mL. 3% BSA from p/n BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody anti-Rabbit IgG was used at 1:5,000. Western Blot of Rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 µg per lane. Primary antibody: CTCF antibody at 1.2 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 3% BSA overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants. 100 μg Log in to see 1 to 2 Days
$389.00
Details
Human Rabbit Un-conjugated ChIP, ICC, IF, IHC, IHC (p), IP, WB Immunohistochemistry-Paraffin: CTCF Antibody [ABIN4300770] - FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CTCF used at a dilution of 1:100 (2ug/ml). Detection: DAB Western Blot: CTCF Antibody [ABIN4300770] - Samples: A) Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CTCF antibody ABIN4300770 lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B). 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IHC, WB Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (40x lens). 100 μL Log in to see 16 Days
$334.02
Details
Bat Rabbit Un-conjugated IHC, IHC (p), WB Human Lung (formalin-fixed, paraffin-embedded) stained with CTCF antibody ABIN214093 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. Human Skin (formalin-fixed, paraffin-embedded) stained with CTCF antibody ABIN214093 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. 50 μg Log in to see 7 to 9 Days
$727.83
Details
Human Rabbit Un-conjugated ELISA, IHC, IHC (p), WB Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 7 to 9 Days
$484.00
Details

Top referenced anti-CTCF Antibodies

  1. Human Monoclonal CTCF Primary Antibody for ELISA, WB - ABIN969071 : Grbesa, Marinkovic, Ivkic, Kruslin, Novak-Kujundzic, Pegan, Bogdanovic, Bedekovic, Gall-Troselj: Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and Helicobacter pylori infection in laryngeal squamous cell carcinoma. in Journal of molecular medicine (Berlin, Germany) 2008 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal CTCF Primary Antibody for IP - ABIN153008 : Lai, Fatemi, Dhasarathy, Malone, Sobol, Geigerman, Jaye, Mav, Shah, Li, Wade: DNA methylation prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas. in The Journal of experimental medicine 2010 (PubMed)

  3. Human Monoclonal CTCF Primary Antibody for ELISA, WB - ABIN965942 : Majumder, Gomez, Chadwick, Boss: The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions. in The Journal of experimental medicine 2008 (PubMed)
    Show all 3 Pubmed References

  4. Human Polyclonal CTCF Primary Antibody for ChIP, ICC - ABIN4300770 : Martínez, Cruz, Lu, Plasschaert, Deng, Rivera-Molina, Bartolomei, Lieberman, Tang: CTCF binding to the first intron of the major immediate early (MIE) gene of human cytomegalovirus (HCMV) negatively regulates MIE gene expression and HCMV replication. in Journal of virology 2014 (PubMed)

  5. Human Polyclonal CTCF Primary Antibody for ChIPSeq, ChIP - ABIN2668282 : Moriguchi, Yu, Takai, Hayashi, Satoh, Suzuki, Ohneda, Yamamoto: The Human GATA1 Gene Retains a 5' Insulator That Maintains Chromosomal Architecture and GATA1 Expression Levels in Splenic Erythroblasts. in Molecular and cellular biology 2015 (PubMed)

More Antibodies against CTCF Interaction Partners

Zebrafish CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) interaction partners

  1. We propose that cohesin and CTCF have distinct functions in the regulation of runx1 (show RUNX1 Antibodies) during zebrafish embryogenesis.

  2. Data conclude that CTCF modulates MRF (show C11orf9 Antibodies) functional interactions in the orchestration of myogenesis.

Mouse (Murine) CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) interaction partners

  1. CCCTC-binding factor CTCF supports the homeostatic maintenance of hematopoietic stem cell (HSCs) pools by sustaining HSC (show FUT1 Antibodies) quiescence in a reactive oxygen species ROS (show ROS1 Antibodies)-dependent manner.

  2. Authors show that mice devoid of an inducible CTCF binding element, located in the alpha constant gene, display a marked isotype-specific increase of GL transcription in developing and resting splenic B cells and altered CSR (show SCARA3 Antibodies) in activated B cells.

  3. The study provides evidence that CTCF regulates chromatin organization during spermiogenesis, contributing to the functional organization of mature sperm.

  4. CTCF Binding Sites in the Herpes Simplex Virus 1 Genome Display Site-Specific CTCF Occupation, Protein Recruitment, and Insulator Function.

  5. Both LDB1 (show LDB1 Antibodies) and CTCF are required for enhancer-Car2 (show CA2 Antibodies) looping, and the domain of LDB1 (show LDB1 Antibodies) contacted by CTCF is necessary to rescue Car2 (show CA2 Antibodies) transcription in LDB1 (show LDB1 Antibodies)-deficient cells.

  6. CTCF physically associates with Wdr5 (show WDR5 Antibodies) and further transcriptionally controls its expression by directly targeting the Wdr5 (show WDR5 Antibodies) gene promoter.

  7. Since CTCF and cohesin are required for loop domain formation, our results suggest that chromatin loops are dynamic and frequently break and reform throughout the cell cycle.

  8. Nearly half of all DNase (show DNASE2 Antibodies) hypersensitivity sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 (show RUNX2 Antibodies) and/or the chromatin-related CTCF transcription factors.

  9. Chromatin immunoprecipitation-DNA sequence analysis was performed in adult cerebellum and Wiz (show ZNF803 Antibodies) peaks were found at promoters and transcription factor CTCF binding sites.

  10. CTCF knockdown attenuates fear-conditioning-induced hippocampal gene expression of key learning genes and loss of long-range interactions at the BDNF (show BDNF Antibodies) and Arc (show NOL3 Antibodies) loci.

Human CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) interaction partners

  1. This study confirms that haploinsufficiency of CTCF causes distinct clinical features, and that a microdeletion encompassing CTCF could cause a recognisable CTCF deletion syndrome. Perturbed DNA methylation (show HELLS Antibodies) at CTCF binding sites, not at imprinted loci, may underlie the pathomechanism of the syndrome.

  2. structural studies show that the sequence-specific interactions between zinc fingers and CTCF-binding sites determine the directionality and conservation of CTCF recognition.

  3. CTCF may be a key factor that contributes to gene co-mutations in cancer.

  4. results support a model in which YY1 (show YY1 Antibodies) acts as an architectural protein to connect developmentally regulated looping interactions; the location of YY1 (show YY1 Antibodies)-mediated interactions may be demarcated in development by a preexisting topological framework created by constitutive CTCF-mediated interactions.

  5. The MeCP2, a protein whose mutated forms are involved in Rett syndrome; and CTCF, a constitutive transcriptional insulator.

  6. The results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL (show WAPAL Antibodies).

  7. CTCF-FOXM1 (show FOXM1 Antibodies) axis regulates tumour growth and metastasis in hepatocellular carcinoma cells.

  8. we show CTCF binding site mutations to be functional by demonstrating allele-specific reduction of CTCF binding to mutant alleles. While topologically associating domains with mutated CTCF anchors in melanoma contain differentially expressed cancer-associated genes, CTCF motif mutations appear generally under neutral selection

  9. CTCF-mediated long-range interactions are integral for a multitude of topological features of interphase chromatin, such as the formation of topologically associated domains, domain insulation, enhancer blocking and even enhancer function.

  10. Authors have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cancer.

CTCF Antigen Profile

Protein Summary

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with CTCF

  • CCCTC-binding factor (CTCF) antibody
  • CCCTC-binding factor (zinc finger protein) (ctcf) antibody
  • CCCTC-binding factor (ctcf) antibody
  • CCCTC-binding factor (Ctcf) antibody
  • AW108038 antibody
  • CTCF antibody
  • im:7152666 antibody
  • xctcf antibody

Protein level used designations for CTCF

transcriptional repressor CTCF , CCCTC-binding factor (zinc finger protein) , transcriptional repressor CTCF-like , 11-zinc finger protein , CTCFL paralog , 11 zinc finger transcriptional repressor

GENE ID SPECIES
100053363 Equus caballus
415104 Danio rerio
454169 Pan troglodytes
100101689 Xenopus (Silurana) tropicalis
100224119 Taeniopygia guttata
100350641 Oryctolagus cuniculus
100465401 Ailuropoda melanoleuca
13018 Mus musculus
10664 Homo sapiens
396274 Gallus gallus
479684 Canis lupus familiaris
517097 Bos taurus
83726 Rattus norvegicus
100623274 Sus scrofa
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