CTCF antibody (C-Term)

Catalog No. ABIN2777204

The Rabbit Polyclonal anti-CTCF antibody (ABIN2777204) specifically detects CTCF in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Rabbit, Pig and Guinea Pig samples.

Quick Overview for CTCF antibody (C-Term) (ABIN2777204)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CTCF Antibody

Binding Specificity: C-Term

Purpose: CTCF Antibody - C-terminal region

Sequence: HADNCAGPDG VEGENGGETK KSKRGRKRKM RSKKEDSSDS ENAEPDLDDN

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CTCF. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human CTCF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31080-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF

Background:

Background Information: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.

Alternative Symbols: MRD21, FAP108, CFAP108

Protein Name: Transcriptional repressor CTCF

Molecular Weight: 83kDa

Gene ID: 10664

NCBI Accession: NP_006556

UniProt: P49711