CTCF antibody

Catalog No. ABIN931147

Product Details anti-CTCF Antibody

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Immunocytochemistry (ICC), Dot Blot (DB)

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: CTCF antibody was raised in mouse using recombinant Human Ccctc-Binding Factor (Zinc Finger Protein)

Clone: 252C3a

Isotype: IgG1

Application Details

Application Notes: ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: CTCF antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF (CTCF Products)

Synonyms: im:7152666 antibody, CTCF antibody, xctcf antibody, AW108038 antibody, CCCTC-binding factor antibody, CCCTC-binding factor (zinc finger protein) antibody, CTCF antibody, ctcf antibody, Ctcf antibody

Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Synonyms: Monoclonal CTCF antibody, Anti-CTCF antibody, CCCTC-binding factor (zinc finger protein) antibody.