anti-Histidyl-tRNA Synthetase (HARS) Antibodies

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Additionally we are shipping Histidyl-tRNA Synthetase Proteins (18) and Histidyl-tRNA Synthetase Kits (8) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HARS 3035 P12081
HARS 15115 Q61035
HARS 307492  
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Top anti-Histidyl-tRNA Synthetase Antibodies at

Showing 10 out of 68 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HARS AntibodyTitration: 1.0 µg/mL  Positive Control: 293T Whole Cell  HARS is supported by BioGPS gene expression data to be expressed in HEK293T 100 μL 2 to 3 Days
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of HARS staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of HARS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL 13 to 14 Days
Human Rabbit Un-conjugated IF, ELISA, WB Western blot analysis of extracts from COLO cells, using HARS Antibody. The lane on the right is treated with the synthesized peptide. Immunofluorescence analysis of HepG2/HeLa cells, using HARS Antibody. The picture on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
Human Rabbit Un-conjugated ELISA, IF, IHC, IHC (p), WB Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE) 50 μL 11 to 14 Days
Human Mouse Un-conjugated IF, WB Western Blot analysis of HARS expression in transfected 293T cell line by HARS MaxPab polyclonal antibody.Lane 1: HARS transfected lysate(55.99 KDa).Lane 2: Non-transfected lysate. HARS MaxPab polyclonal antibody. Western Blot analysis of HARS expression in human liver. 50 μL 11 to 12 Days
Human Rabbit Un-conjugated ELISA, IF, IHC, WB 100 μL Available
Cow Rabbit Un-conjugated WB 100 μL 11 to 14 Days
Human Rabbit Un-conjugated ELISA, ICC, IF, WB Western blot analysis of extracts from rat brain, using HARS Antibody. ABIN6274166 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. 100 μL 11 to 12 Days
Human Rabbit Un-conjugated EIA, IHC (p), WB Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane).HARS (arrow) was detected using the purified Pab (1:60 dilution). 0.4 mL 6 to 8 Days
Human Mouse Un-conjugated ELISA, WB HARS monoclonal antibody (M03), clone 4D4. Western Blot analysis of HARS expression in HeLa . Detection limit for recombinant GST tagged HARS is approximately 0.3ng/ml as a capture antibody. 50 μg 11 to 12 Days

Top referenced anti-Histidyl-tRNA Synthetase Antibodies

  1. Human Polyclonal HARS Primary Antibody for ELISA, WB - ABIN543930 : Lu, Hope, Brasch, Reinhard, Cohen: TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation. in Proceedings of the National Academy of Sciences of the United States of America 2003 (PubMed)
    Show all 2 Pubmed References

  2. Human Polyclonal HARS Primary Antibody for ELISA, WB - ABIN561225 : Chardonnet, Decottignies, Amar, Le Caer, Davis, Laroche, Le Maréchal: New mortalin and histidyl tRNA synthetase isoforms point out a pitfall in proteomic analysis of Egr1 genetically modified mice. in Proteomics 2007 (PubMed)

More Antibodies against Histidyl-tRNA Synthetase Interaction Partners

Human Histidyl-tRNA Synthetase (HARS) interaction partners

  1. the first biochemical analysis of Charcot-Marie-Tooth-associated HARS mutations and underscores how loss of the primary aminoacylation function can contribute to disease pathology.

  2. This study suggests that the human HisRS genes, while descending from a common ancestor with dual function for both types of tRNA(His), have acquired highly specialized tRNA recognition properties through evolution.

  3. Despite the similar kinetics, differential scanning fluorimetry revealed that Y454S is less thermally stable than Wild Type HARS, and cells from Y454S patients grown at elevated temperatures demonstrate diminished levels of protein synthesis compared to those of Wild Type cells. The thermal sensitivity associated with the Y454S mutation represents a biochemical basis for understanding Usher Syndrome Type IIIB.

  4. Loss of function mutations in histidyl-tRNA synthetase cause a spectrum of inherited peripheral neuropathies

  5. Data suggest that by comparing human and trypanosomatid histidyl-tRNA synthetases (HisRS) may provide opportunities for developing specific inhibitors of Trypanosoma brucei HisRS.

  6. Secreted histidyl-tRNA synthetase splice variants elaborate major epitopes for autoantibodies in inflammatory myositis.

  7. Data indicate that higher anti-Jo1 levels were associated with disease severity in antisynthetase syndrome (ASS) patients.

  8. Non-Jo-1 anti-synAb positive patients have decreased survival compared with Jo-1 patients.

  9. Findings suggest that histidyl-tRNA synthetase (HARS) is associated with axonal peripheral neuropathy.

  10. The crystal structure of a novel splice variant of a tRNA synthetase lacking the entire catalytic domain.

  11. Although anti-Jo1 positive patients with antisynthetase syndrome share features of patients with anti-PL7/PL12 antibody, they exhibit many differences regarding clinical phenotype and long-term outcome.

  12. Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS.

  13. genomic organization of the HARS locus and mapping of transcripts originating from a bi-directional promoter controlling the differential expression of these gene

  14. Demonstrating histidyl-tRNA synthetase (Jo-1)-specific T cell responses represents a key step in establishing the hypothesis that Jo-1 drives T cell-mediated autoimmunity in Jo-1+ polymyositis.

  15. the TSG101 interaction with HRS is a crucial step in endocytic down-regulation of mitogenic signaling and this interaction may have a role in linking the functions of early and late endosomes

  16. A proteolytically sensitive conformation of HisRS exists in the lung, the target tissue associated with this autoantibody response.

  17. clinical and prognostic profiles of 45 patients displaying anti-histidyl-tRNA synthetase autoantibodies were determined

Mouse (Murine) Histidyl-tRNA Synthetase (HARS) interaction partners

  1. AA 60-90 of HRS were absolutely required for in vitro as well as in vivo signaling via MyD88-dependent TLR pathways to case myositis.

Histidyl-tRNA Synthetase (HARS) Antigen Profile

Protein Summary

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with HARS

  • histidyl-tRNA synthetase (HARS) antibody
  • histidyl-tRNA synthetase (Hars) antibody
  • Dnd1 antibody
  • HARSL antibody
  • HRS antibody
  • MMHRS antibody
  • USH3B antibody

Protein level used designations for HARS

HisRS , histidine translase , histidine--tRNA ligase, cytoplasmic , hisRS , histidine tRNA ligase , histidyl-tRNA synthetase, cytoplasmic , dead end homolog 1 , Histidyl-tRNA synthetase, cytoplasmic

478034 Canis lupus familiaris
3035 Homo sapiens
15115 Mus musculus
307492 Rattus norvegicus
510937 Bos taurus
100173931 Pongo abelii
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