anti-Mitochondrial Calcium Uptake 1 (MICU1) Antibodies

Human Mitochondrial Calcium Uptake 1 (MICU1) interaction partners

1. Authors propose that MICU1 has to interact with the D-ring formed by the DIME domains in MCU to control the uniporter.

2. FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation

3. drives aberrant metabolism that fuels aerobic glycolysis and chemoresistance in ovarian cancer

4. the absence of MICU1, both Mn(2+) and Ca(2+) can pass through the uniporter, as evidenced by mitochondrial Mn(2+) uptake assays, mitochondrial membrane potential measurements, and mitoplast electrophysiology.

5. Mitochondrial Ca(2+) uptake is controlled by protein arginine methyl transferase 1 that asymmetrically methylates MICU1, resulting in decreased Ca(2+) sensitivity. UCP2/3 normalize Ca(2+) sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca(2+) uptake activity.

6. MICU2 restricts spatial crosstalk between InsP3R and MCU channels by regulating threshold and gain of MICU1-mediated inhibition and activation of MCU.

7. study concludes that cooperative, high-affinity interaction of the MICU1-MICU2 complex with Ca(2+) serves as an on-off switch, leading to a tightly controlled channel, capable of responding directly to cytosolic Ca(2+) signals

8. MICU1 serves as a signal-noise discriminator in mitochondrial calcium signalling, limiting the energetic costs of mitochondrial Ca2+ signalling which may undermine oxidative phosphorylation, especially in tissues with highly dynamic energetic demands.

9. Results showed that the SNP on EF-hand Ca2+ binding domains of MICU1 gene had no effect in phenotypic characters of bipolar disorder patients

10. Our experiments provide novel details about how MCU/EMRE is regulated by MICU1 and an original approach to investigate MCU/EMRE activation in intact cells.

11. Data show that ribosomal protein S3 (RPS3) knockdown decreased mitochondrial calcium uptake 1 protein (MICU1) expression.

12. Loss of heterozygosity of MICU1 gene on chromosome 10q is associated with pancreatic cancer.

13. Expression of MICU1 mutants lacking functional Ca2+-binding sites leads to a striking loss of Ca2+ uptake in HEK293 cells.

14. MICU1 EF hands determine MCU activity, but not binding.

15. MICU1 and MICU2 have roles in tuning the mitochondrial Ca2+ uniporter by exerting opposite effects on MCU activity

16. Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.

17. Prolonged MICU1 loss leads to an adaptive increase in matrix Ca(2+) binding, yet cells show impaired oxidative metabolism and sensitization to Ca(2+) overload.

18. CBARA1 is a marker for undifferentiated human embryonic stem cells that plays a role in maintaining stemness, cell cycle progression, and proliferation.

19. MICU1 is a novel regulator of the machinery in cancer cells that prevents apoptosis.

20. MICU1 is a gatekeeper of mitochondrial calcium uniporter-mediated Ca(2 )uptake that is essential to prevent [Ca(2 )]overload and associated stress.

Mouse (Murine) Mitochondrial Calcium Uptake 1 (MICU1) interaction partners

1. Authors propose that MICU1 has to interact with the D-ring formed by the DIME domains in MCU to control the uniporter.

2. s. We propose that in the MICU1-MICU2 oligomeric complex the C-terminal helices of both proteins form a central semiautonomous assembly which contributes to the gating mechanism of the uniporter.

3. FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation

4. MICU1 exerted protection against myocardial ischemia/reperfusion injury via prevention of mitochondrial Ca2+ overload, preservation of mitochondrial integrity and promotion of mitochondrial energy metabolism.

5. that MICU1 serves as a molecular gatekeeper preventing calcium overload and suggests that modulating the calcium uniporter could have widespread therapeutic benefits

6. a possible role for tissue-specific stoichiometry between the Ca(2+)-sensing regulators (MICUs) and pore unit (MCU) of the uniporter, was investigated.

7. A MICU1 splice variant confers high sensitivity to the mitochondrial Ca(2+) uptake machinery of skeletal muscle.

8. MICU1 was downregulated in db/db mouse hearts, which contributes to myocardial apoptosis in diabetes. Importantly, the reconstitution of MICU1 in diabetic hearts significantly inhibited the development of diabetic cardiomyopathy, as evidenced by enhanced cardiac function and reduced cardiac hypertrophy and myocardial fibrosis in db/db mice.

9. MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and liver regeneration.