Phospho p38 Flex Set (Bead B6)

Catalog No. ABIN1379679

Quick Overview for Phospho p38 Flex Set (Bead B6) (ABIN1379679)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Application: Immunoassay (IA), Flow Cytometry (FACS)

Product Details

Binding Specificity: pThr180, pTyr182

Reactivity: Human, Mouse, Rat

Brand: BD™ Cytometric Bead Array,BD™

Characteristics: The BD™ CBA Phospho p38 (T180/Y182) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat p38, a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-180 and tyrosine-phosphorylated at Tyr-182 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho p38 (T180/Y182) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho p38 (T180/Y182) Flex Set. The biology and function of p38 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

Components: Capture beads, detection reagent, standards

Application Details

Comment:

Bead Position: B6

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

References

Boldt, Kolch: "Targeting MAPK signalling: Prometheus' fire or Pandora's box?" in: Current pharmaceutical design, Vol. 10, Issue 16, pp. 1885-905, (2004) (PubMed).

Yang, Sharrocks, Whitmarsh: "Transcriptional regulation by the MAP kinase signaling cascades." in: Gene, Vol. 320, pp. 3-21, (2003) (PubMed).

Target Details

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: p38