Streptavidin Resin

Catalog No. ABIN1536312

Quick Overview for Streptavidin Resin (ABIN1536312)

Target: Streptavidin

Application: Purification (Purif), Affinity Chromatography (AC)

Bead Ligand: Streptavidin

Bead Matrix: Agarose beads

Bead Size: 90 µm

Product Details

Purpose: Streptavidin Resin is an affinity chromatography medium designed for easy, one-step purification of biotinylated peptides, antibodies, lectins, etc from samples.

Specificity: Resin Volume 5 ml settled resin (10 ml 50% slurry)
Ligand Streptavidin, affinity purified with IminoBiotin Resin (L00272)
Number of biotin binding sites per ligand 4
M.W. of ligand Approximately 54 kDa
PI of ligand 6.52
Degree of substitution Approximately 2 mg streptavidin/ml settled resin
Total binding capacity > 120 nmol of D-Biotin/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45 - 165 μm)
Storage solution 1X PBS containing 20% ethanol
Storage conditions 2 - 8°C
Shelf life 12 months when stored unopened

Characteristics: The purified recombinant streptavidin is covalently coupling to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for biotinylated molecules. The total binding capacity of Streptavidin Resin is more than 120 nmol of D-Biotin/ml settled resin. 

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1X PBS containing 20% ethanol

Handling Advice: High temperature heating is not recommended. The agarose melts above 65°C.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Streptavidin

Background: Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an extremely high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to where most useful biological interactions occur. As a result, streptavidin frequently exhibits much lower non-specific binding than avidin does. 

Molecular Weight: ~ 54 kDa