Turbo-I First Strand cDNA Synthesis Kit

Catalog No. ABIN1543649

Quick Overview for Turbo-I First Strand cDNA Synthesis Kit (ABIN1543649)

Product Details

Characteristics: Turbo-I Reverse Transcriptase is a new generation reverse transcriptase, the best of its kind in the market. The enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA or DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.Furthermore, Turbo-I Reverse Transcriptase contains an additional fidelity-enhancing subunit which drastically enhances accuracy in reverse transcription. Turbo-I First Strand cDNA Synthesis Kit contains all components required for first strand cDNA synthesis, with the choice of using either Oligo(dT) and/or Random Primers. The Oligo(dT) anneals selectively to the Poly(A) tail of mRNAs. Random Primers do not require the presence of poly (A) and can be used for the transcription of mRNA 5’-end regions. Gene-specific primers may also be used with the kit. The recombinant Rnase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. The first strand cDNA can be directly used as a template in PCR.
One unit is defined as the amount of enzyme required to incorporate 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C using Poly (A) and Oligo(dT) as template and primer, respectively.

Components: Turbo-I Reverse Transcriptase ( 200 U/µL): 25 µL
Oligo(dT)(10 µM): 40 µL
Random Primers (10 µM): 40 µL
dNTPs (10 mM): 40 µL
Rnase Inhibitor (40 U/µL) : 15 µL
5x RT Buffer: 150 µL
Nuclease-free H2O: 1 mL

Application Details

Application Notes: Oligo(dT) are oligonucleotides that anneal to the 3’ - Poly(A) + mRNA. Therefore, only mRNA or total RNA templates with 3’ - Poly(A) tails are used in cDNA synthesis. Random Primers are oligonucleotides that anneal at non - specific sites of RNA templates. Therefore, all forms of RNA can be used in cDNA synthesis. Gene - Specific Primers are oligonucleotides that are designed to anneal to the specific site of a target gene.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01 % (v/v) NP-40, 50 % (v/v) glycerol.

Storage: -20 °C

Storage Comment: Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly.

Expiry Date: 12 months