Mouse TrueBlot® Anti-Mouse Ig Biotin

Catalog No. ABIN1589968

Supplier Product No.: 13-8817-82

Quick Overview for Mouse TrueBlot® Anti-Mouse Ig Biotin (ABIN1589968)

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Supplier Product No.: 13-8817-82

Supplier: Rockland

Brand: TrueBlot®

Characteristics: Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blotting with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot® ULTRA is the biotinylated format of Mouse IgG TrueBlot® ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
Conjugation Name: Biotin TrueBlot® ULTRA

Components: Mouse TrueBlot®: Anti-Mouse Ig Biotin

Application Details

Application Notes: Western Blot Dilution: 1:2000

Comment:

Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations:
1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate.
2. Use milk powder for complete and effective blocking of the western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: Proprietary

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.

Expiry Date: 6 months

References

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