Anti-DYKDDDDK G1 Affinity Resin

Catalog No. ABIN2017617

Quick Overview for Anti-DYKDDDDK G1 Affinity Resin (ABIN2017617)

Target: DYKDDDDK Tag

Application: Immunoprecipitation (IP), Purification (Purif), Separation (Sep)

Product Details

Reactivity: Tag

Clonality: Monoclonal

Specificity: High DYKDDDDK-tag binding capacity and ultralow levels of non-specific binding
Multiple elution methods for diverse protein characteristics and different requirements
Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Characteristics: DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.
Matrix: 4 % cross-linked agarose
Average bead size: 90 μm
Ligand: Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity: Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin

Application Details

Comment:

Resin reuse: The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method: Acid, alkaline, neutral, peptide competitive and SDS-PAGE loading buffer elutions
Reagents compatibility: Compatible with commonly used reagents at certain concentrations

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 % settled resin in TBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze the resin.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for up to 12 months.

Expiry Date: 12 months

Target Details

Target: DYKDDDDK Tag

Alternative Name: DYKDDDDK

Target Type: Tag