Monofinity A Resin Prepacked Column
Catalog No. ABIN2017618
Quick Overview for Monofinity A Resin Prepacked Column (ABIN2017618)
Application
Immunoprecipitation (IP), Separation (Sep)
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Brand
- Monofinity A Resin™
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Specificity
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High binding capacity : >50 mg IgG/mL settled resin
High recovery rate : >97 % in average
Enhanced stability : good thermal and chemical stability
Low ligand leakage : < 15 ng/mg IgG
Reliable scale-up : highly suitable for large scale antibody purification -
Characteristics
- Protein A affinity chromatography resin is the most commonly applied method for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus. It has five high affinity binding sites capable of binding specifically to the Fc region of immunoglobulin molecules from several species. Covalently immobilized Protein A matrices have been extensively used to purify IgG from several species of mammals. Monofinity A Resin is made of recombinant protein A as ligand, which not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also enhances chemical stability under Cleaning-in-place (CIP) conditions. The typical dynamic binding capacity is more than 50 g human IgG/L settled resin. There is no significant decrease of binding capacity after purification/CIP for 200 cycles. In the meanwhile, the Monofinity A Resin has a low leakage of ligand (< 15 ng ligand/mg IgG) over a number of purification/CIP cycles. As a high quality antibody purification resin, it is highly suitable for large scale antibody purification, and meets the purification requirements of industry-scale customers.
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Comment
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Monofinity A Resin™ is made of a novel recombinant protein A ligand that has a high specific binding capacity to the Fc region of immunoglobulin molecules and enhances chemical stability under Cleaning-in-Place (CIP) conditions. Monofinity A Resin™ has several benefits such as high-flow agarose base, low non-specific binding and better dynamic binding capacity than similar products.
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Restrictions
- For Research Use only
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Format
- Liquid
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Handling Advice
- Do not freeze.
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Storage
- 4 °C
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Storage Comment
- Store the Monofinity A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C.
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