Human CD8 T Lymphocyte Enrichment Set-DM

Catalog No. ABIN2690971

Quick Overview for Human CD8 T Lymphocyte Enrichment Set-DM (ABIN2690971)

Target: CD8

Product Details

Reactivity: Human

Brand: BD IMag™

Application Details

Comment:

BD IMag™ Human CD8 T Lymphocyte Enrichment Set-DM

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. 557941 Rev. 1 Page 1 of 3 Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD™ IMag Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD™ IMagnet (Cat. No. 552311) according to the accompanying Protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC-conjugated anti-human CD8 mAb RPA-T8 (Cat. No. 555369) to detect CD8 T lymphocytes and a mixture of PE-conjugated RPA-T4 (Cat. No. 555347), ICRF44 (Cat. No. 555388), 3G8 (Cat. No. 555407), HIB19 (Cat. No. 555413), CB38 (NL07) (Cat. No. 555455), B159 (Cat. No. 555516), 7G3 (Cat. No. 554529), GA-R2 (HIR2) (Cat. No. 555570), and B1 (Cat. No. 555717) monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes. Dead cells were excluded by staining with propidium iodide. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Target Details

Target: CD8