Mitochondrial Antigen
-
- Application
- ELISA
- Characteristics
- Mitochondrial antigen M2 is a cluster of four major mitochondrial innermembrane proteins of approximately 74, 52, 45 and 39 kDa. Contains the E2 subunit of branched chain 2-oxo acid dehydrogenase, the E2 subunit of the 2-oxo dehydrogenase complex, protein X and the E1a and E1b subunits of the pyruvate dehydrogenase complex. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with ELISA assays.
- Purification
- Affinity Purified using immobilized human anti-Mitochondrial antibodies.
-
-
- Application Notes
- Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.01M Tris containing 0.53M Sodium chloride
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
- Handling Advice
- Avoid multiple freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -70 °C + 10 °C.
-