Human Buffy Coat Preparation
Quick Overview for Human Buffy Coat Preparation (ABIN925536)
Host
Lysate Type
Protein Source
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Supplier Product No.
- r614-0100
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Supplier
- Rockland
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Specificity
- Buffy coat preparations are composed of leukocytes, trace amounts of red blood cells and other components
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Characteristics
- Strain: Adult
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Sterility
- Non-sterile
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Components
- Human Buffy Coat Preparation
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Rat Tail
Host: Rat
Guinea Pig Spinal CordHost: Guinea Pig Normal Spinal Cord
Sheep Red Blood Cells (10% Washed Pooled Cells)H Host: Sheep Normal Red Blood Cells
Mouse BrainIEM, IHC, WB Host: Mouse Normal Brain
Chicken Red Blood Cells (10% Washed Pooled Cells)H Host: Chicken Normal Red Blood Cells
Hamster Complement (Lyophilized) With DiluentHost: Hamster Normal Complement
Guinea Pig Complement (Lyophilized) With DiluentHost: Guinea Pig Normal Complement
Rabbit Blood (In Sodium Citrate)H Host: Rabbit Normal Blood
Sheep Red Blood Cells (100% Washed Pooled Cells)H Host: Sheep Normal Red Blood Cells
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Application Notes
- Buffy coat preparations are suitable for use as a source of nucleated PBMC. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). Buffy coat preparations are generally enriched for leukocytes (WBC) and contain trace amounts of red cells and other blood components. If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll™ gradient centrifugation. This product is prepared in a buffer composed of 20.5 mg/ml Dextrose, 4.2mg/ml Sodium Chloride, 8.0 mg/ml Citric Acid Tri-sodium, adjusted to pH 6.1.
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Comment
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Blood is a complex mixture of cells, proteins, metabolites and many other substances. About 56% of human blood volume is comprised of cells, more than 99% of which are erythrocytes. Human erythrocytes and thrombocytes (platelets, 0.5% blood components) do not contain nuclei. Leukocytes (0.3% of blood components), also referred to as peripheral blood mononuclear leukocytes or PBMC, do contain nuclei. Blood samples may vary widely in leukocyte (granulocytes, lymphocytes and monocytes) concentration depending on the health of the donor. Healthy blood contains less than 107 leukocytes per ml, while blood from an infected donor may have ten fold higher leukocyte concentrations.
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Restrictions
- For Research Use only
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Format
- Frozen
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Handling Advice
- For extended storage, aliquot contents to minimize freeze/thaw cycles.
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Storage
- -80 °C
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Expiry Date
- 12 months
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