AGO5 antibody
This Rabbit Polyclonal antibody specifically detects AGO5 in IF, IP and WB. It exhibits reactivity toward Arabidopsis thaliana.
Catalog No. ABIN1720795
$646.39
Plus shipping costs $50.00
50 μg
Shipping to:
United States
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Quick Overview for AGO5 antibody (ABIN1720795)
Target
AGO5
(Argonaute Family Protein (AGO5))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Purpose
- Anti-AGO5 | Argonaute 5
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Specificity
- TAIR: At2g27880
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Cross-Reactivity (Details)
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Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Populus sp., Solanum lycopersicum, Zea mays -
Predicted Reactivity
- Arabidopsis thaliana
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO5 protein sequence
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Application Notes
- 1 : 100 (IF), 5 μg per gram floral tissue (IP), 1 : 1500 (WB)
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Comment
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AGO5 (Argonaute Family Protein (AGO5))
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Alternative Name
- AGO5
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Background
- AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
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Molecular Weight
- 111 | 111 kDa
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Gene ID
- 817334
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UniProt
- Q9SJK3
Target
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