GFP antibody (Green Fluorescent Protein) (AA 246)

Details for Product anti-GFP Antibody No. ABIN100085
Antigen
Epitope
AA 246
19
16
9
7
5
4
2
2
2
2
1
1
1
1
Reactivity
Aequorea victoria
399
10
10
9
9
7
6
3
2
1
Host
Goat
168
155
57
29
16
7
3
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
19
16
12
9
6
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
342
195
108
87
83
54
27
25
22
20
15
12
10
8
8
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1
1
1
Options
Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name GFP
Background Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
UniProt P42212
Application Notes Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Comment

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) Western Blot of Rabbit anti-GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfecte...
Image no. 2 for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to ...
Image no. 3 for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) Western Blot of Goat anti-GFP antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK...
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