HIF3A antibody (AA 575-600)

Catalog No. ABIN100475

Supplier Product No.: 600-401-435

The Rabbit Polyclonal anti-HIF3A antibody has been validated for WB and ELISA. It is suitable to detect HIF3A in samples from Mouse. There is 1 publication available.

Quick Overview for HIF3A antibody (AA 575-600) (ABIN100475)

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HIF3A Antibody

Binding Specificity: AA 575-600

Supplier Product No.: 600-401-435

Supplier: Rockland

Purpose: Hif3 alpha Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against mouse Hif3alpha protein.

Characteristics: Synonyms: rabbit anti-Hif3a antibody, Hif3 alpha antibody, Hif3α antibody, Hypoxia Inducible Factor 3-alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Inhibitory PAS domain protein antibody, IPAS antibody, HIF-3-alpha, Basic-helix-loop-helix-PAS protein MOP7, HIF3-alpha-1, Neonatal and embryonic PAS protein

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.

Western Blot Dilution: 1:1,000 - 1:8,000

ELISA Dilution: 1:14,000 - 1:80,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-HIF3A antibody (ABIN100475)

Takeda, Ho, Takeda, Duan, Nagy, Fong: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, (2006) (PubMed).

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: Hif3a

Background: Background: One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF binds to the hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-a may be a marker for tumor growth and apoptosis. Hif3 may participate in disorders with the cornea, lung, and heart. Anti-Hif3 alpha Antibody is useful for researchers interested in transcription factors, DNA binding, and Epilepsy research.

Gene ID: 53417, 170295859

UniProt: Q0VBL6

Pathways: Warburg Effect