Huntingtin antibody (HTT) (AA 416-424)

Details for Product anti-HTT Antibody No. ABIN100604
Antigen
Epitope
AA 416-424, pSer421
13
9
7
6
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
132
79
66
9
6
3
3
2
2
2
2
2
2
2
2
2
1
1
Host
Rabbit
88
51
3
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
98
68
52
30
18
16
13
13
10
8
5
2
2
2
1
Options
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 416-424 of Human huntingtin protein.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name Huntington (HTT Antibody Abstract)
Background Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons.  It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization.  This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Synonyms: AI256365 antibody, C430023I11Rik antibody, HD antibody, HD protein antibody, HDH antibody, HTT antibody, HUNTINGTON CHOREA antibody, Huntington disease protein antibody, Huntingtin protein antibody, IT15,
Gene ID 3064
UniProt P42858
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport
Application Notes Anti-Huntingtin pS421 antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at approximately 350 kDa and 200 kDa in size corresponding to full-length Huntingtin protein and truncated (hypothetical) Huntingtin protein, respectively, by western blotting in the appropriate cell lysate or extract.   This antibody is specific for the phosphorylated form of Huntingtin protein at the pS421 residue.  The identity of lower molecular bands ~130 kDa is not known.
Restrictions For Research Use only
Format Liquid
Concentration 0.6 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (ABIN100604) Western blot analysis after AKT and phosphatase treatment is shown using Affinity Pu...
Image no. 2 for anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (ABIN100604) Western blot analysis is shown using Affinity Purified anti-Huntingtin pS421 antibod...