Rabbit anti-Cow IgG Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN100939

Supplier Product No.: 601-4503

Product Details

Target: IgG

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 601-4503

Supplier: Rockland

Purpose: Bovine IgG Fc Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG, Bovine Serum and Bovine F(c) and no reaction against Bovine F(ab)2.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Bovine IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Bovine F(c) Alkaline Phosphatase Conjugate has been tested by ELISA. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

, Preservative:0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.