Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (FITC)

Catalog No. ABIN101297

Supplier Product No.: 206-4202

Quick Overview for Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (FITC) (ABIN101297)

Target: IgG

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Guinea Pig

Host: Rabbit

Supplier Product No.: 206-4202

Supplier: Rockland

Purpose: Guinea Pig IgG (H&L) Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum.

Characteristics: Anti-Guinea Pig Red Blood Cells Antibody recognizes Guinea Pig red blood cells and can be used in a variety of agglutination assays where agglutination or clumping of red blood cells is a positive indication of the presence of an analyte, virus particle or bacteria. Red blood cells (RBCs), also known as erythrocytes, are metabolically active cells that are highly adapted to serve their function in blood gas exchange (oxygen/CO2 transport). The red blood cells enable the transport of sufficient O2 between respiratory surfaces (lungs, gills) and metabolizing tissues by means of their high intracellular concentration of hemoglobin.

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Guinea Pig IgG whole molecule

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.