Goat anti-Horse IgG (Fc Region) Antibody

Catalog No. ABIN101444

Supplier Product No.: 608-1103

Quick Overview for Goat anti-Horse IgG (Fc Region) Antibody (ABIN101444)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Horse

Host: Goat

Supplier Product No.: 608-1103

Supplier: Rockland

Purpose: Horse IgG Fc Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum.  No reaction was observed against Horse IgG F(ab).

Characteristics: Anti-Horse IgG Antibody generated in goat detects horse IgG.

Purification: Anti-Horse IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Horse IgG F(c) fragment

Application Details

Application Notes: Application Note: Anti-Horse IgG F(c) antibody has been tested by ELISA and dot blot and is suitable for Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Horse IgG F(c) antibody is ideal for investigators involved in serum component protein research.