Gelsolin antibody (N-Term)

Catalog No. ABIN1019662

This Goat Polyclonal antibody specifically detects Gelsolin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Gelsolin antibody (N-Term) (ABIN1019662)

Target: Gelsolin (GSN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Gelsolin Antibody

Binding Specificity: N-Term

Purpose: Gelsolin (isoform a)

Sequence: PQGRVPEARP NS

Specificity: This antibody is expected to recognize isoform a (NP_000168.1) only . Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1,

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PQGRVPEARPNS-C, from the N Terminus of the protein sequence according to NP_000168.1, NP_937895.1, NP_001121138.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human Colon, Duodenum, Heart and Skeletal Muscle lysates (calculated MW of 85.7 kDa according to NP_000168.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN

Background: GSN, gelsolin, ADF, AGEL, DKFZp313L0718, OTTHUMP00000022011, OTTHUMP00000022014, OTTHUMP00000162202, actin-depolymerizing factor, brevin

Gene ID: 2934

NCBI Accession: NP_000168, NP_937895, NP_001121138

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy