Donkey anti-Rabbit IgG (Fc Region) Antibody

Catalog No. ABIN102057

Supplier Product No.: 111-7103

Quick Overview for Donkey anti-Rabbit IgG (Fc Region) Antibody (ABIN102057)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Fc Region

Reactivity: Rabbit

Host: Donkey

Supplier Product No.: 111-7103

Supplier: Rockland

Purpose: Rabbit IgG Fc Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-donkey serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab).

Characteristics: Anti-Rabbit IgM antibody generated in goat specifically detects rabbit IgM mu heavy chain.

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Rabbit IgG F(c) fragment

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Antibody Anti-Rabbit IgG F(c) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 80 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.