EGFR antibody (PE)
(4 references)-
- Target See all EGFR Antibodies
- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Reactivity
- Human, Horse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EGFR antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1), epitope within amino acids 6-273.
- No Cross-Reactivity
- Mouse, Rat
- Cross-Reactivity (Details)
- Human, Equine (Horse)
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Human epidermoid carcinoma line A431
- Clone
- EGFR1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product EGFR Primary Antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep WB Host: Goat Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibodyEGFR Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Rat WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- Comment
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).
: "Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations." in: Journal of cell science, Vol. 116, Issue Pt 19, pp. 4043-52, (2003) (PubMed).
: "Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation." in: Oncogene, Vol. 20, Issue 17, pp. 2101-11, (2001) (PubMed).
: "Changing patterns of localization of putative stem cells in developing human hair follicles." in: The Journal of investigative dermatology, Vol. 114, Issue 2, pp. 321-7, (2000) (PubMed).
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: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).
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- Target
- EGFR (Epidermal Growth Factor Receptor (EGFR))
- Alternative Name
- EGFR (EGFR Products)
- Background
- Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGF-R, Her1, ERBB1, PIG61, NISBD2
- Gene ID
- 1956
- UniProt
- P00533
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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