CENPA antibody

Catalog No. ABIN1027707

This anti-CENPA antibody is a Mouse Monoclonal antibody detecting CENPA in WB, IF and ICC. Suitable for Human.

Quick Overview for CENPA antibody (ABIN1027707)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5A7-2E11

Product Details anti-CENPA Antibody

Specificity: Detects ~18 kDa. Recognizes Human CENP-A.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Synthetic peptide corresponding to a portion of human CENP-A

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1027707 was sufficient for detection of CENPA in 20 μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA

Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Gene ID: 1058

NCBI Accession: NP_001035891

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location