SLC17A7 antibody (AA 493-560)
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- Target See all SLC17A7 Antibodies
- SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))
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Binding Specificity
- AA 493-560
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SLC17A7 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Immunohistochemistry (mIHC)
- Specificity
- Detects ~52 kDa. No cross-reactivity against VGlut2.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
- Clone
- S28-9
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN1027710 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- by
- Akoya Biosciences
- No.
- #104338
- Date
- 04/20/2021
- Antigen
- SLC17A7
- Lot Number
- 1205
- Method validated
- Multiplex Immunohistochemistry
- Positive Control
Fresh frozen mouse olfactory bulb
- Negative Control
Unlabeled control (mouse fresh frozen)
- Notes
Passed. The anti-SLC17A7 antibody ABIN1027710 labels areas of high glutamatergic afferent input throughout the murine olfactory bulb with a concentration in the internal plexiform and granule cell layer.
- Primary Antibody
- ABIN1027710
- Secondary Antibody
- Full Protocol
- Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
- Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
- Conjugation of the anti-SLC17A7 antibody ABIN1027710 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.
- Experimental Notes
No signal was detected in unlabeled specimens.
Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.
Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
Validation #104338 (Multiplex Immunohistochemistry)Validation ImagesMurine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647).Full Methods -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))
- Alternative Name
- VGLUT1 (SLC17A7 Products)
- Synonyms
- 2900052E22Rik antibody, AI851913 antibody, VGluT1 antibody, Vglut1 antibody, BNPI antibody, VGLUT1 antibody, slc17a7 antibody, vglut1 antibody, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody, solute carrier family 17 member 7 antibody, solute carrier family 17 (vesicular glutamate transporter), member 7a antibody, Slc17a7 antibody, SLC17A7 antibody, slc17a7a antibody
- Background
- VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
- Gene ID
- 116638
- NCBI Accession
- NP_446311
- UniProt
- Q62634
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