Sucrase Isomaltase, Regulatory (SI-R) antibody (Biotin)

Catalog No. ABIN103307

Supplier Product No.: 200-106-189-0100

This anti- antibody is a Goat Polyclonal antibody detecting in ELISA and WB. Suitable for Candida sp..

Quick Overview for Sucrase Isomaltase, Regulatory (SI-R) antibody (Biotin) (ABIN103307)

Target: Sucrase Isomaltase, Regulatory (SI-R)

Reactivity: Candida sp.

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Product Details

Supplier Product No.: 200-106-189-0100

Supplier: Rockland

Purpose: Invertase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Invertase [Candida species].

Characteristics: Synonyms: goat anti-Invertase Antibody biotin Conjugated, Biotin Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody

Purification: Anti-Invertase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Invertase [Candida species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Invertase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Invertase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Sucrase Isomaltase, Regulatory (SI-R)

Alternative Name: SUC1

Background: Background: Anti-Invertase antibody recognizes invertase which hydrolyzes the alpha-1,4-glycosidic bond between alpha-D-glucose and beta-D-fructose of sucrose. Bees use invertase to convert nectar to honey. In the confectionary industry, yeast-derived invertase is used to hydrolyze sucrose to glucose and fructose as fructose is more sweeter and less prone to crystallization. Anti-Invertase antibody is ideal for investigators interested in Metabolism and Cell Biology.

Gene ID: 3640338

UniProt: P10594