alpha 2 Macroglobulin antibody

Catalog No. ABIN103762

Supplier Product No.: 200-101-207-0100

This Goat Polyclonal antibody specifically detects alpha 2 Macroglobulin in WB, IHC and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for alpha 2 Macroglobulin antibody (ABIN103762)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-alpha 2 Macroglobulin Antibody

Supplier Product No.: 200-101-207-0100

Supplier: Rockland

Purpose: Alpha-2-Macroglobulin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma].

Characteristics: Synonyms: goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Purification: Anti-alpha-2-MACROGLOBULIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: a2-Macroglobulin [Human Plasma]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-alpha-2-macroglobulin antibody has been tested by western blot and is suitable for ELISA and Immunohistochemistry applications. Anti-alpha-2-MACROGLOBULIN antibody should be optimized by the end user for the specific reactive conditions.

Western Blot Dilution: 1:2,000 - 1:20,000

ELISA Dilution: 1:80,000 - 1:400,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-alpha 2 Macroglobulin antibody (ABIN103762)

Chung, Hsu, Chen, Liu, Chang, Li, Huang, Shieh, Lee: "Association of salivary alpha 2-macroglobulin levels and clinical characteristics in type 2 diabetes." in: Journal of diabetes investigation, Vol. 7, Issue 2, pp. 190-6, (2017) (PubMed).

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2M

Background: Background: Alpha-2-Macroglobulin detects Macroglobulin. Alpha-2-MACROGLOBULIN is a large plasma protein found in the blood. It is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. Alpha 2-Macroglobulin is the largest major nonimmunoglobulin protein in plasma. The alpha 2-macroglobulin molecule is synthesized mainly in liver, but also locally by macrophages, fibroblasts, and adrenocortical cells. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha 2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of Alpha 2 macroglobulin. The concentration of alpha 2 macroglobulin rises 10-fold or more in the nephrotic syndrome when other lower molecular weight proteins are lost in the urine. The loss of alpha 2 macroglobulin into urine is prevented by its large size. The net result is that alpha 2 macroglobulin reaches serum levels equal to or greater than those of albumin in the nephrotic syndrome, which has the effect of maintaining oncotic pressure. Antialpha-2-macroglobulin is ideal for investigators involved in serum component protein research.

Gene ID: 2

NCBI Accession: NP_000005

UniProt: P01023

Pathways: Lipid Metabolism