Maltose Phosphorylase antibody

Catalog No. ABIN103916

Supplier Product No.: 200-101-230-0100

The Goat Polyclonal anti-Maltose Phosphorylase antibody has been validated for WB, IP and ELISA. It is suitable to detect Maltose Phosphorylase in samples from E. coli.

Quick Overview for Maltose Phosphorylase antibody (ABIN103916)

Target: Maltose Phosphorylase

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This Maltose Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-Maltose Phosphorylase Antibody

Supplier Product No.: 200-101-230-0100

Supplier: Rockland

Purpose: Maltose Phosphorylase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.

Characteristics: Synonyms: goat anti-Maltose Phosphorylase Antibody, mapA antibody, mpA antibody

Purification: Anti-MALTOSE PHOSPHORYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Maltose Phosphorylase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Maltose Phosphorylase has been tested by western blot. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. 

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Maltose Phosphorylase

Target: Maltose Phosphorylase

Background: Background: Maltose Phosphorylase catalyzes the phosphorolysis of maltose, leading to the formation of glucose and glucose 1-P.

Gene ID: 6233598

NCBI Accession: YP_001842841

UniProt: B2GEX2