Histone H4 antibody (meLys20)

Catalog No. ABIN1042605

The Mouse Monoclonal anti-Histone H4 antibody (Clone 5E10-D8) (ABIN1042605) specifically detects Histone H4 in WB, IF, ELISA, ICC, ChIP, DB, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for Histone H4 antibody (meLys20) (ABIN1042605)

Target: Histone H4 (HISTH4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 5E10-D8

Product Details anti-Histone H4 Antibody

Binding Specificity: meLys20

Specificity: Specific for K20 mono-methylated histone H4, no cross-reactivity with K20 tri-, di-, or unmethylated histone H4, no cross-reactivity with histone H3.

Cross-Reactivity: Arabidopsis thaliana, Drosophila melanogaster, Mammalian, Xenopus laevis

Cross-Reactivity (Details): Human. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals

Purification: Protein G

Immunogen: Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched))

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try, WB: Use a concentration of 1 μg/mL. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).Can be blocked with Histone H4 peptide - mono methyl K20 

Comment:

Myeloma, fusion partners: X63-Ag8.653

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Synonyms:
H4F2 antibody,H4FN antibody,HIST1H4 antibody,HIST2H4 antibody,HISTH4H4 antibody,methyl histone H4 antibody,methylated histone H4 antibody

Gene ID: 318846, 3771854, 3771893

OMIM: 142750

UniProt: P59259, P84040, P02304, P62805, P62799