DYKDDDDK Tag antibody

Catalog No. ABIN1043727

Supplier Product No.: 200-301-b13

This anti-DYKDDDDK Tag antibody is a Mouse Monoclonal antibody detecting DYKDDDDK Tag in WB, ELISA, IHC and FACS. Suitable for . This Primary Antibody has been cited in 2+ publications.

Quick Overview for DYKDDDDK Tag antibody (ABIN1043727)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 29E4-G7

Product Details anti-DYKDDDDK Tag Antibody

Supplier Product No.: 200-301-b13

Supplier: Rockland

Purpose: DYKDDDDK Tag (Anti-FLAG®) Antibody

Sequence: DYKDDDDK

Cross-Reactivity (Details): The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins.

Characteristics: Synonyms: mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Purification: This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG2a kappa

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Anti-FLAG antibody has been tested by ELISA and western blot and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:150,000 - 1:250,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-DYKDDDDK Tag antibody (ABIN1043727)

Huang, Chuang, Hao, Talbot, Sen, Trink, Sidransky, Ratovitski: "Phospho-?Np63? is a key regulator of the cisplatin-induced microRNAome in cancer cells." in: Cell death and differentiation, Vol. 18, Issue 7, pp. 1220-30, (2011) (PubMed).

Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, (1996) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: FLAG

Target Type: Tag

Background: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.