PDCD4 antibody (pSer457)

Catalog No. ABIN1043728

Supplier Product No.: 200-301-b17

Product Details anti-PDCD4 Antibody

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Binding Specificity: pSer457

Reactivity: Human, Mouse, Rat, Arthropods

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-301-b17

Supplier: Rockland

Purpose: Pdcd4 phospho S457 Antibody

Cross-Reactivity (Details): This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.

Characteristics: Synonyms: mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Purification: Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Pdcd4 phospho S457 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Immunogen Type: Conjugated Peptide

Clone: 9G6

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 1.25-2.5 μg/mL

Application Note: Anti-PDCD4 pS457 monoclonal antibody has been tested by ELISA and western blotting and is suitable for immunohistochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:1,000-1:3,000

ELISA Dilution: 1:400,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Pdcd4 phospho S457 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PDCD4 antibody (ABIN1043728)

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4 (PDCD4 Products)

Background: Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.

Gene ID: 27250, 21735596

UniProt: Q53EL6