Interleukin 17a antibody (Biotin)

Catalog No. ABIN1043792

Supplier Product No.: 209-306-b32

The Mouse Monoclonal anti-Interleukin 17a antibody has been validated for ELISA, WB and FACS. It is suitable to detect Interleukin 17a in samples from Human. There is 1 publication available.

Quick Overview for Interleukin 17a antibody (Biotin) (ABIN1043792)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4H1524-1

Product Details anti-Interleukin 17a Antibody (Biotin)

Supplier Product No.: 209-306-b32

Supplier: Rockland

Purpose: IL-17A Biotin Conjugated Antibody

Cross-Reactivity (Details): This antibody is specific for human IL-17A protein.

Characteristics: Synonyms: mouse anti-Interleukin-17A biotin conjugated antibody, mouse anti-IL-17A biotin conjugated antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17

Purification: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG2b

Labeling Ratio: 10-20

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: This purified antibody has been tested by Western Blot and suitable for use in FLOW. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Interleukin 17a antibody (Biotin) (ABIN1043792)

Paust, Turner, Steinmetz, Peters, Heymann, Hölscher, Wolf, Kurts, Mittrücker, Stahl, Panzer: "The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 5, pp. 969-79, (2009) (PubMed).

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A

Background: Background: IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Gene ID: 3605

NCBI Accession: NP_002181

UniProt: Q16552