CCL20 antibody (Biotin)

Catalog No. ABIN1043793

Supplier Product No.: 209-306-b51

This anti-CCL20 antibody is a Mouse Monoclonal antibody detecting CCL20 in WB. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CCL20 antibody (Biotin) (ABIN1043793)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL20 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: 3N2D9

Product Details anti-CCL20 Antibody (Biotin)

Supplier Product No.: 209-306-b51

Supplier: Rockland

Purpose: MIP-3 alpha Biotin Conjugated Antibody

Cross-Reactivity (Details): This antibody is specific for human MIP-3α protein.

Characteristics: Synonyms: mouse anti-MIP-3 alpha Antibody biotin Conjugated, mouse anti-MIP-3a biotin Conjugated Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Purification: This product was purified from mouse ascites by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-MIP-3alpha (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human MIP-3alpha produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: This purified antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.5 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CCL20 antibody (Biotin) (ABIN1043793)

Lisignoli, Manferdini, Codeluppi, Piacentini, Grassi, Cattini, Filardo, Facchini: "CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups." in: Journal of cellular physiology, Vol. 221, Issue 1, pp. 154-60, (2009) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background: Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Gene ID: 6364, 4759076

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection