CCL20 antibody (HRP)

Catalog No. ABIN1043828

Supplier Product No.: 210-403-b51

Product Details anti-CCL20 Antibody (HRP)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL20 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 210-403-b51

Supplier: Rockland

Purpose: MIP-3 alpha Antibody Peroxidase Conjugated

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-MIP-3α peroxidase conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Purification: Mouse MIP-3 alpha Antibody Peroxidase Conjugated was heated to 56°C for 30 minutes.

Immunogen:

Immunogen: MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3alpha protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This purified antibody has been tested in western blotting and is suitable for ELISA. By western blot a band approximately 10.7 kDa in size corresponding to mouse MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store MIP-3 alpha Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CCL20 antibody (HRP) (ABIN1043828)

Harper, Guo, Rizzo, Lillis, Kurtz, Skorcheva, Purdy, Fitch, Iordanov, Blauvelt: "Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis." in: The Journal of investigative dermatology, Vol. 129, Issue 9, pp. 2175-83, (2009) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: Ccl20 (CCL20 Products)

Background: Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Gene ID: 20297

NCBI Accession: NP_058656

UniProt: O89093

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection