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IL-27 antibody (Biotin)

The Rabbit Polyclonal anti-IL-27 antibody has been validated for WB, ELISA and IHC. It is suitable to detect IL-27 in samples from Mouse. There is 1 publication available.
Rockland
Catalog No. ABIN1043831
Supplier Product No.: 210-406-b54

Quick Overview for IL-27 antibody (Biotin) (ABIN1043831)

Target

See all IL-27 (IL27) Antibodies
IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity

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Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This IL-27 antibody is conjugated to Biotin

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Supplier Product No.

    210-406-b54

    Supplier

    Rockland

    Purpose

    IL-27/p28 Antibody Biotin Conjugated

    Cross-Reactivity (Details)

    This antibody is specific for mouse IL-27/p28 protein.

    Characteristics

    Synonyms: rabbit anti-IL-27/p28 biotin conjugated antibody, rabbit anti-interleukin 27a biotin conjugated antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

    Purification

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.

    Immunogen Type: Recombinant Protein

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kDa in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract.

    Western Blot Dilution: 1:2,000-1:10,000

    ELISA Dilution: 1:20,000-1:100,000

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Expiry Date

    12 months
  • Pflanz, Timans, Cheung, Rosales, Kanzler, Gilbert, Hibbert, Churakova, Travis, Vaisberg, Blumenschein, Mattson, Wagner, To, Zurawski, McClanahan, Gorman, Bazan, de Waal Malefyt, Rennick, Kastelein: "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4+ T cells." in: Immunity, Vol. 16, Issue 6, pp. 779-90, (2002) (PubMed).

  • Target

    IL-27 (IL27) (Interleukin 27 (IL27))

    Alternative Name

    Il27

    Background

    Background: The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.

    Gene ID

    246779

    NCBI Accession

    NP_663611

    UniProt

    Q8K3I6
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