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Mouse anti-Goat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

ELISA, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
Catalog No. ABIN1043938
Supplier Product No.: 605-305-002

Quick Overview for Mouse anti-Goat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN1043938)

Target

IgG

Clonality

  • 3014
  • 142
  • 6
  • 1
Polyclonal

Conjugate

  • 536
  • 362
  • 358
  • 282
  • 226
  • 202
  • 186
  • 108
  • 92
  • 71
  • 57
  • 55
  • 50
  • 50
  • 49
  • 44
  • 43
  • 40
  • 39
  • 39
  • 39
  • 37
  • 31
  • 29
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 27
  • 26
  • 26
  • 26
  • 25
  • 24
  • 24
  • 22
  • 22
  • 22
  • 22
  • 22
  • 22
  • 22
  • 22
  • 21
  • 19
  • 17
  • 17
  • 17
  • 15
Alkaline Phosphatase (AP)

Application

  • 1686
  • 1669
  • 1637
  • 1567
  • 920
  • 873
  • 753
  • 406
  • 345
  • 232
  • 232
  • 106
  • 87
  • 71
  • 57
  • 31
  • 24
  • 19
  • 18
  • 18
  • 16
  • 16
  • 12
  • 9
  • 8
  • 7
  • 5
  • 4
  • 4
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Dot Blot (DB)

Isotype

IgG
  • Binding Specificity

    • 2242
    • 315
    • 286
    • 149
    • 130
    • 67
    • 52
    • 29
    • 28
    • 28
    • 21
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 610
    • 547
    • 529
    • 395
    • 343
    • 231
    • 186
    • 154
    • 130
    • 124
    • 109
    • 86
    • 84
    • 82
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Goat

    Host

    • 1479
    • 1364
    • 523
    • 205
    • 98
    • 83
    • 38
    • 29
    • 21
    • 20
    • 16
    • 11
    • 11
    Mouse

    Supplier Product No.

    605-305-002

    Supplier

    Rockland

    Purpose

    Goat IgG (H&L) (Mouse) Antibody Alkaline Phosphatase Conjugated

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Mouse Serum, Goat IgG and Goat Serum. No reaction was observed against Rabbit, Human and Mouse Serum Proteins.

    Characteristics

    Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification

    Goat IgG (H&L) Antibody Alkaline Phosphatase Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Sterility

    Sterile filtered

    Immunogen

    Optional[Immunogen]: Goat IgG whole molecule
  • Application Notes

    Application Note: Anti-Goat IgG Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

    Expiry Date

    12 months
  • Target

    IgG

    Target Type

    Antibody

    Background

    Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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