Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (Texas Red (TR))

Catalog No. ABIN1044012

Supplier Product No.: 703-409-002

Quick Overview for Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) (ABIN1044012)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Application: Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Rabbit

Supplier Product No.: 703-409-002

Supplier: Rockland

Purpose: F(ab')2 Chicken IgG (H&L) Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG and Chicken Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Characteristics: F(ab')2 Anti-Chicken IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Chicken IgG whole molecule

Application Details

Application Notes: Application Note: F(ab')2 Anti-Chicken IgG Texas Red Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.