NFYA antibody (N-Term)

Catalog No. ABIN104769

Supplier Product No.: 200-401-100

This anti-NFYA antibody is a Rabbit Polyclonal antibody detecting NFYA in WB and ELISA. Suitable for Human and Monkey. This Primary Antibody has been cited in 4+ publications.

Quick Overview for NFYA antibody (N-Term) (ABIN104769)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NFYA Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-401-100

Supplier: Rockland

Purpose: NF-Y Antibody

Cross-Reactivity (Details): A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Characteristics: Synonyms: rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A

Purification: Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NFYA antibody (ABIN104769)

Falcone, Grandoni, Garibaldi, Manni, Filligoi, Piaggio, Gurtner: "Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153658, (2016) (PubMed).

Gurtner, Fuschi, Magi, Colussi, Gaetano, Dobbelstein, Sacchi, Piaggio: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, (2008) (PubMed).

Cecchinelli, Lavra, Rinaldo, Iacovelli, Gurtner, Gasbarri, Ulivieri, Del Prete, Trovato, Piaggio, Bartolazzi, Soddu, Sciacchitano: "Repression of the antiapoptotic molecule galectin-3 by homeodomain-interacting protein kinase 2-activated p53 is required for p53-induced apoptosis." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4746-57, (2006) (PubMed).

Yu, Zhu, Pi, Ling, Ko, Takeda, Tuan: "The long terminal repeat (LTR) of ERV-9 human endogenous retrovirus binds to NF-Y in the assembly of an active LTR enhancer complex NF-Y/MZF1/GATA-2." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35184-94, (2005) (PubMed).

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA

Background: Background: Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.

Gene ID: 4800

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha