ADRA2C antibody (Extracellular Domain)
-
- Target See all ADRA2C Antibodies
- ADRA2C (Adrenergic, alpha-2C-, Receptor (ADRA2C))
-
Binding Specificity
- Extracellular Domain
-
Reactivity
- Human, Monkey
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ADRA2C antibody is un-conjugated
-
Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human ADRA2C. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Panda, Bovine, Dog, Guinea pig (94%) Hamster (89%).
- Purification
- Immunoaffinity purified
- Immunogen
-
Synthetic 18 amino acid peptide from 2nd extracellular domain of human ADRA2C. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Panda, Bovine, Dog, Guinea pig (94%), Hamster (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADRA2C Primary Antibody
-
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (Internal Region) antibody
ADRA2C Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (HRP)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 HRP
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (FITC)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 FITC
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (Biotin)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 Biotin
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (PE)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 PE
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (PerCP)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 PerCP
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (APC)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 APC
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (Atto 594)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 Atto 594
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (Atto 488)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 Atto 488
anti-Adrenergic, alpha-2C-, Receptor (ADRA2C) (AA 442-462) antibody (Atto 390)ADRA2C Reactivity: Human WB, ICC, IF Host: Mouse Monoclonal S330A-80 Atto 390
-
- Application Notes
- Approved: IHC, IHC-P (15 - 30 μg/mL)
- Comment
-
Target Species of Antibody: Human
- Assay Procedure
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- ADRA2C (Adrenergic, alpha-2C-, Receptor (ADRA2C))
- Alternative Name
- ADRA2C (ADRA2C Products)
- Background
-
Name/Gene ID: ADRA2C
Subfamily: Adrenergic
Family: GPCR
Synonyms: ADRA2C, ADRA2RL2, ADRARL2, Alpha-2C adrenergic receptor, Alpha-2c-2 adrenergic receptor, ADRA2L2, Adrenoceptor alpha 2C, Alpha-2C-adrenergic receptor, Alpha-2C-AR, Alpha-2CAR, Alpha-2C adrenoceptor, Alpha-2C adrenoreceptor, Alpha2c, ALPHA2CAR, Alpha 2c, Alpha2-AR-C4, Alpha2cii-adrenergic receptor - Gene ID
- 152
- Pathways
- EGFR Signaling Pathway, cAMP Metabolic Process
-
