RORC antibody (Ligand Binding Domain)

Catalog No. ABIN1049300

The Rabbit Polyclonal anti-RORC antibody is suitable to detect RORC in samples from Human and Monkey. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for RORC antibody (Ligand Binding Domain) (ABIN1049300)

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RORC Antibody

Binding Specificity: Ligand Binding Domain

Specificity: Human ROR Gamma. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RORA (56 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Opossum (100%) Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit (94%) Mouse, Rat, Hamster (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from ligand-binding domain of human ROR Gamma. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Opossum (100%), Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit (94%), Mouse, Rat, Hamster (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (15 - 20 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Alternative Name: RORC / ROR gamma

Background: Name/Gene ID: RORC
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: RORC, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, RORG, RORgamma, TOR, ROR Gamma, RZRG, NR1F3, RZR-GAMMA

Gene ID: 6097

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway