PLG antibody (Biotin)

Catalog No. ABIN105703

Supplier Product No.: 200-106-208-0100

The Goat Polyclonal anti-PLG antibody has been validated for WB and ELISA. It is suitable to detect PLG in samples from Human.

Quick Overview for PLG antibody (Biotin) (ABIN105703)

Target: PLG (Plasminogen (PLG))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLG antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-PLG Antibody (Biotin)

Supplier Product No.: 200-106-208-0100

Supplier: Rockland

Purpose: Plasminogen Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma].

Characteristics: Synonyms: goat anti-Plasminogen Antibody, biotin Conjugated goat anti-Plasminogen Antibody, Angiostatin antibody, Microplasmin antibody, Plasmin antibody, Plasmin heavy chain A antibody, Plasmin light chain B antibody, PLG antibody

Purification: Anti-Plasminogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Plasminogen [Human Plasma]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Plasminogen antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of Plasminogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PLG

Target: PLG (Plasminogen (PLG))

Alternative Name: PLG

Background: Background: Anti-Plasminogen antibody recognizes plasminogen, a precursor of plasmin. Plasmin is a serine protease that degrades fibrin clots and involved in embryonic development, tissue remodeling, tumor invasion, and inflammation. In addition to fibrin, Plasmin can also cleave fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin can activate other zymogens such as tissue plasminogen activator, urokinase plasminogen activator, kallikrein, and factor XII. Deficiency in plasminogen increases susceptibility to thrombosis.

Gene ID: 5340

UniProt: P00747

Pathways: Complement System, Lipid Metabolism