Pyranose Oxidase antibody

Catalog No. ABIN106381

Supplier Product No.: 200-101-235-0100

This Goat Polyclonal antibody specifically detects Pyranose Oxidase in IP, WB and ELISA. It exhibits reactivity toward E. coli.

Quick Overview for Pyranose Oxidase antibody (ABIN106381)

Target: Pyranose Oxidase

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This Pyranose Oxidase antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), ELISA

Product Details anti-Pyranose Oxidase Antibody

Supplier Product No.: 200-101-235-0100

Supplier: Rockland

Purpose: Pyranose Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.

Characteristics: Synonyms: goat anti-Pyranose Oxidase Antibody, P2Ox, Pyranose oxidase, PROD, POD, POx, Pyranose:oxygen 2-oxidoreductase, Glucose 2-oxidase, FAD-oxidoreductase

Purification: Anti-Pyranose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Pyranose Oxidase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Pyranose Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Pyranose Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Pyranose Oxidase

Target: Pyranose Oxidase

Background: Background: Pyranose Oxidase catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O2 to H2O2. It plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H2O2 for the ligninolytic peroxidases, lignin peroxidase, and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.

UniProt: P79076