Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RFX5 antibody

Rockland 200-401-194 RFX5 Reactivity: Human WB, ELISA, GS, ChIP Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN106544
Supplier Product No.: 200-401-194
  • Target See all RFX5 Antibodies
    RFX5 (Regulatory Factor X 5 (RFX5))
    Reactivity
    • 29
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Human
    Host
    • 26
    • 3
    • 1
    Rabbit
    Clonality
    • 28
    • 2
    Polyclonal
    Conjugate
    • 26
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFX5 antibody is un-conjugated
    Application
    • 28
    • 17
    • 6
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Gel Shift (GS), Chromatin Immunoprecipitation (ChIP)
    Supplier Product No.
    200-401-194
    Supplier
    Rockland
    Purpose
    RFX5 Antibody
    Cross-Reactivity (Details)
    Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.
    Characteristics
    Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody
    Purification
    RFXF5 antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product RFX5 Primary Antibody
  • Application Notes

    Gel Shift Dilution: 0.5 μL to 1.0 μL

    Application Note: Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG.  Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5.  This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay.

    ChIP Dilution: User Optimized

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Zhao, Xie, Liao, Zhang, Cao, Fei, Wang, Wei, Shao, Chen: "The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma." in: Oncology reports, Vol. 37, Issue 1, pp. 289-296, (2017) (PubMed).

    Dille, Kleinschnitz, Kontchou, Nölke, Häcker: "In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport." in: Infection and immunity, Vol. 83, Issue 8, pp. 3268-80, (2015) (PubMed).

    Xu, Sengupta, Seto, Smith: "Regulatory factor for X-box family proteins differentially interact with histone deacetylases to repress collagen alpha2(I) gene (COL1A2) expression." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9260-70, (2006) (PubMed).

  • Target
    RFX5 (Regulatory Factor X 5 (RFX5))
    Alternative Name
    RFX5 (RFX5 Products)
    Background
    Background: Anti-RFX5 is specific for the RFX5 protein. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA.
    Gene ID
    5993
    UniProt
    P48382
You are here:
Support