RFX5 antibody (C-Term)

Catalog No. ABIN106545

Supplier Product No.: 200-401-191

This anti-RFX5 antibody is a Rabbit Polyclonal antibody detecting RFX5 in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for RFX5 antibody (C-Term) (ABIN106545)

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RFX5 Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-401-191

Supplier: Rockland

Purpose: RFX5 Antibody

Cross-Reactivity (Details): Anti-RFX5 (C-terminal specific) may react with a minor band at 68 kDa which may represent an RFX5 breakdown product or unmodified RFX5.

Characteristics: Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

Purification: This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: RFX5 (C-terminal specific) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This product was assayed by immunoblot and found to be reactive against RFX5 (C-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:250 to 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-RFX5 (C-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band.  This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay. 

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RFX5 antibody (ABIN106545)

Chen, Chai, Hart, Zhong: "Identifying catalytic residues in CPAF, a Chlamydia-secreted protease." in: Archives of biochemistry and biophysics, Vol. 485, Issue 1, pp. 16-23, (2009) (PubMed).

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5

Background: Background: DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.

Gene ID: 5993

UniProt: P48382