Fibronectin antibody (AA 313-607)

Catalog No. ABIN1077665

The Rabbit Polyclonal anti-Fibronectin antibody has been validated for WB, IHC, IP, FACS and ICC. It is suitable to detect Fibronectin in samples from Human. There are 3+ publications available.

Quick Overview for Fibronectin antibody (AA 313-607) (ABIN1077665)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Fibronectin Antibody

Binding Specificity: AA 313-607

Purpose: Polyclonal Antibody to Fibronectin (FN)

Specificity: The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Fibronectin (FN)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.32 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-Fibronectin antibody (ABIN1077665)

Lu, Chen, Liu, Si, Zhao, Lai, Kang, Sun, Guo: "Molecular hydrogen regulates PTEN-AKT-mTOR signaling via ROS to alleviate peritoneal dialysis-related peritoneal fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 34, Issue 3, pp. 4134-4146, (2021) (PubMed).

Nishad, Mukhi, Tahaseen, Mungamuri, Pasupulati: "Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy." in: The Journal of biological chemistry, Vol. 294, Issue 44, pp. 16109-16122, (2020) (PubMed).

Zheng, Hu, Huang, Xu, Yang, Li: "In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study." in: Biomedical materials (Bristol, England), Vol. 10, Issue 1, pp. 015016, (2015) (PubMed).

Target Details for Fibronectin

Target: Fibronectin

Background: FN1, CIG, FINC, LETS, MSF, GFND2, Anastellin, Migration-Stimulating Factor, Cold-Insoluble Globulin, Large, External, Transformation-Sensitive Protein