POMC antibody (AA 27-235)

Catalog No. ABIN1077682

This Rabbit Polyclonal antibody specifically detects POMC in WB, IHC, IP and ICC. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for POMC antibody (AA 27-235) (ABIN1077682)

Target: POMC (Proopiomelanocortin (POMC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-POMC Antibody

Binding Specificity: AA 27-235

Purpose: Polyclonal Antibody to Proopiomelanocortin (POMC)

Specificity: The antibody is a rabbit polyclonal antibody raised against POMC. It has been selected for its ability to recognize POMC in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB311Mu01Recombinant Proopiomelanocortin (POMC)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-POMC antibody (ABIN1077682)

Muhsin, Bentley, Bai, Goldsworthy, Cox: "A novel mutation in the mouse Pcsk1 gene showing obesity and diabetes." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 31, Issue 1-2, pp. 17-29, (2020) (PubMed).

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background: POC, ProOMC, Pro-OMC, Pro Opiomelanocortin, Corticotropin-lipotropin

Molecular Weight: 25 kDa

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour