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Prothrombin antibody (AA 44-200)

This anti-Prothrombin antibody is a Rabbit Polyclonal antibody detecting Prothrombin in WB, IHC, IP and ICC. Suitable for Mouse.
Catalog No. ABIN1077942

Quick Overview for Prothrombin antibody (AA 44-200) (ABIN1077942)

Target

See all Prothrombin (F2) Antibodies
Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity

  • 56
  • 29
  • 23
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Mouse

Host

  • 59
  • 26
  • 7
  • 4
  • 2
  • 1
  • 1
Rabbit

Clonality

  • 73
  • 27
Polyclonal

Conjugate

  • 70
  • 14
  • 8
  • 2
  • 2
  • 2
  • 2
This Prothrombin antibody is un-conjugated

Application

  • 75
  • 52
  • 40
  • 28
  • 24
  • 14
  • 11
  • 10
  • 9
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 44-200

    Purpose

    Polyclonal Antibody to Coagulation Factor II (F2)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against F2. It has been selected for its ability to recognize F2 in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    RPA820Mu01Recombinant Coagulation Factor II (F2)

    Isotype

    IgG
  • Application Notes

    Western blottin:g: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

    Alternative Name

    Coagulation Factor II

    Background

    FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2

    Pathways

    Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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